A Rapid Assay to Screen for Drug‐Resistant Herpes Simplex Virus

A rapid assay to screen for drug-resistant herpes simplex virus.

A rapid assay was developed to screen for herpes simplex virus (HSV) isolates that are resistant to acyclovir and other antiviral agents. The assay is a modified plaque reduction assay (PRA) in which the number of plaques seen in the absence of acyclovir was compared with that seen in the presence of a single cutoff concentration of acyclovir (2 microg/mL). This assay utilizes a cell line that ...

Rapid detection of herpes simplex virus.

was developed to detect herpes simplex virus (HSV) antigen. Rabbit anti-HSV antibody (immunoglobulin G fraction) was coated on flat-bottom, irradiated, 96-well polystyrene microtiter plates and served to capture HSV antigen. Clinical specimens from patients with genital herpes were added. Biotin-linked rabbit anti-HSV immunoglobulin G was used as the second antibody. The antigen-antibody comple...

Herpes Simplex Virus Meningoencephalitis in A Methamphetamin User

Herpes Simplex Virus and Langerhans Cell Histiocytosis

Background and objective: Langerhans cell histiocytosis (LCH) is a rare histiocytic proliferative disorder of unknown etiology and mainly affects young children. The histological feature is granuloma-like proliferation of langerhans-type dendritic cells. Although the possible role of viruses such as Epstein-Barr virus (EBV, Human Herpes virus -4</...

Rapid amplified enzyme linked immunosorbent assay evaluated for detecting herpes simplex virus.

A new amplified enzyme linked immunosorbent assay (amplified ELISA) kit for detecting herpes simplex virus (HSV) antigen was evaluated. Duplicate swabs were taken from 180 patients with clinically suspected herpes lesions. Tests were performed on a direct swab extract and viral transport medium containing a swab. Of the 93 culture positive specimens, 78 of the extracted samples (sensitivity 83....